Search results for "Degalactosylated xyloglucan"

showing 4 items of 4 documents

In-situ gelling xyloglucan formulations as 3D artificial niche for adipose stem cell spheroids.

2020

Abstract Three-dimensional spheroidal cell aggregates of adipose stem cells (SASCs) are a distinct upstream population of stem cells present in adipose tissue, with enhanced regeneration properties in vivo. The preservation of the 3D structure of the cells, from extraction to administration, can be a promising strategy to ensure optimal conditions for cell viability and maintenance of stemness potential. With this aim, an artificial niche was created by incorporating the spheroids into an injectable, in-situ gelling solution of partially degalactosylated xyloglucan (dXG) and an ad hoc formulated culture medium for the preservation of stem cell spheroid features. The evolution of the mechani…

Cell SurvivalPopulationCellCell Culture TechniquesAdipose tissue02 engineering and technology[object Object]Biochemistry03 medical and health scienceschemistry.chemical_compoundStructural BiologySpheroids CellularmedicineHumansViability assayeducationMolecular BiologyGlucansCells Cultured030304 developmental biology0303 health scienceseducation.field_of_studyMicroscopyTissue EngineeringViscosityRegeneration (biology)SOXB1 Transcription FactorsSpheroids of adipose stem cells Artificial niche In-situ forming gel Partially degalactosylated xyloglucanSpheroidHydrogelsMesenchymal Stem CellsGeneral MedicineNanog Homeobox Protein021001 nanoscience & nanotechnologyCell biologyCulture MediaXyloglucanmedicine.anatomical_structurechemistryMicroscopy Electron ScanningXylansSettore CHIM/07 - Fondamenti Chimici Delle TecnologieStem cell0210 nano-technologyRheologyShear StrengthOctamer Transcription Factor-3International journal of biological macromolecules
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SELF-ASSEMBLY OF DEGALACTOSYLATED XYLOGLUCAN FROM TAMARIND SEEDS

2014

Degalactosylated xyloglucan
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Injectable hydrogel formulations to host adipose stem cell spheroids for stemness maintenance and bone and cartilage regeneration

2021

Adipose-derived mesenchymal stem cells (ASCs) represent a great promise for tissue regeneration as fat is a very abundant source of stem cells (1) and owing to their ability to self-renew and differentiate into specific tissue types (2). In general, they are usually cultured as two-dimensional (2D) adherent monolayers, not representative of the in vivo condition, often entailing lower cell viability and, more in general, a lower “cell quality” in terms of regenerative potential (3, 4). When ASCs are cultured in low adhesion flasks and with a suitable culture medium, they aggregate in the form of three-dimensional spheroids (SASCs). The incorporation of these spheroids into injectable, in-si…

Settore CHIM/07 - Fondamenti Chimici Delle TecnologieSpheroids of adipose stem cells artificial niche in-situ forming gel partially degalactosylated xyloglucan injectable hydrogels
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Gelation of degalactosylated xyloglucan from nano to macroscopic scale

2016

Partially degalactosylated xyloglucans are able to form thermo-reversible gels in aqueous solution at physiological temperature. This property makes them very promising materials for tissue engineering. Moreover, due to a highly branched, hydroxyl group-rich molecular structure, xyloglucans are particularly interesting for manufacturing of micro/nanoparticles to be used as nano-scalar delivery devices of active ingredients in cosmetic and pharmaceutical formulations. Here we present results on the temperature-induced self-assembly of degalactosylated xyloglucan at varying polymer concentration from dilute to semi-dilute regime. Our aim is to investigate the gelation mechanism for gaining in…

degalactosylated xyloglucans gelationSettore CHIM/07 - Fondamenti Chimici Delle Tecnologie
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